Page 65 - Portuguese Journal - SPORL - Vol 61. Nº1

(3 weeks), and tricyclic antidepressants (1 week) 19-20 . a) Conditions of the test room
The solution used in the NAPT is composed of an The patients should be adjusted to the
allergen extract at a predefined concentration, conditions of the room where the NAPT will be
diluted in an isotonic solution at neutral pH 19- performed for 15-30 minutes before the start of
18
20 . Rondón et al. established a NAPT protocol the test. The room should be at a temperature
with multiple allergens, in which they used the of 20+/-1.5 ºC with humidity between 40% and
following concentrations of the aeroallergens 60% 15,19-20 .
most commonly associated with LAR: solutions
of Dermatophagoides pteronyssinus at 4 µm/ b) Qualified staff and emergency medication
mL, Alternaria alternata at 0.25 µm/mL, Olea The patients should sign an informed consent
europaea at 0.6 µm/mL, and grasses at 0.1 µm/ form before undergoing the NAPT. The site
mL. of the test should be located at less than a
30-minute distance from an emergency room
4.2 Protocol for the NAPT (Figure 2): and intensive care unit. There should be an
Certain conditions need to be met for performing emergency medicine kit in the room .
19
the NAPT to ensure its reproducibility and
similarity with daily life conditions:

Figure 2
Protocol of the Nasal Allergen Provocation Test (NAPT)

Subjective parameters: symptoms reported by the patient (nasal congestion, rhinorrhea, sneezing, nasal itching, ocular symptoms),
classified according to their severity (0 – none; 1 – mild; 2 – moderate; 3 – severe). Objective parameters: evaluation of nasal permeability
through active anterior rhinomanometry.

Volume 61 . Nº1 . March 2023 65